InBase: the Intein Database
نویسنده
چکیده
InBase, the Intein Database (http://www.neb.com/neb/inteins.html ), is a comprehensive on-line resource that includes the Intein Registry. Inteins are protein splicing elements that mediate a self-catalytic protein splicing reaction. InBase presents general information as well as detailed data for each intein, including tabu-lated comparisons and a comprehensive bibliography.
منابع مشابه
InBase, the New England Biolabs Intein Database
Inteins are intervening sequences that splice as proteins, not RNA. InBase, the New England Biolabs Intein Database (http://www.neb. com/neb/inteins.html), is a comprehensive on-line database that includes the Intein Registry, along with detailed information about each intein and its host protein, tabulated comparisons and a comprehensive bibliography including papers in press.
متن کاملCompilation and analysis of intein sequences.
We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the 36 available intein sequences refines the previously described intein motifs and reveals the presence of another intein motif, Block H. Furthermore, analysis of the new inteins reshapes our view of ...
متن کاملDesign and Construction of Recombinant ELP-Intein Cassette for Use in Simple and new Purification Methods of Recombinant Proteins
Background and Objective: Use of elastin-like proteins (ELPs) provides high-performance protein purification without need for chromatography. In line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from ELP b...
متن کاملهمسانهسازی و بیان ایمونوتوکسین اونتاک به صورت هیبریدی با دنباله اینتئینی
Introduction: Inteins (INT) are internal parts of a number of proteins in yeast and some other unicellular eukaryotes, which can be separated from the immature protein during protein splicing process. After identifying the mechanism of intein action, applications of these sequences are be considered in the single- step purification of recombinant proteins and different intein tags were develope...
متن کاملAn Evolved Mxe GyrA Intein for Enhanced Production of Fusion Proteins
Expressing antibodies as fusions to the non-self-cleaving Mxe GyrA intein enables site-specific, carboxy-terminal chemical modification of the antibodies by expressed protein ligation (EPL). Bacterial antibody-intein fusion protein expression platforms typically yield insoluble inclusion bodies that require refolding to obtain active antibody-intein fusion proteins. Previously, we demonstrated ...
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 30 1 شماره
صفحات -
تاریخ انتشار 2000